Review



easysep human nk cell isolation kit  (STEMCELL Technologies Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    STEMCELL Technologies Inc easysep human nk cell isolation kit
    A) Flow cytometry showing GD2 expression levels in the GD2 low cell lines BE2C, NGP and NB1 treated for 14 days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. B) Mean fluorescence intensity for GD2 staining shown in panel A. C-D) Gene expression levels (in TPM from RNA-seq) of B4GALNT1 (C) and ST8SIA1 (D) in BE2C cells treated for six days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. Shown are three biological replicates, and the values represent the mean ± SD. Significance was determined by one-way ANOVA. E) Immunofluorescence for GD2 from FFPE tissue sections of BE2C xenografts in the indicated treatment groups obtained on day 14 of treatment. Scale bar = 20 μm. F-I) Cell viability of BE2C and NB1 cells following both 1) treatment with either DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363 for 14 days and subsequent 2) co-culture with F-G) <t>NK</t> <t>cells</t> and dinutuximab for 24 hours or H-I) GD2 CAR T cells at an effector-to-target (E:T) ratio of 0.5:1 for 48 hours ( n =3 samples per treatment group). Data represent mean values ± SD. Significance was determined by one-way ANOVA. *p<0.05; **p <0.001; ***p <0.001; ****p <0.0001. Each compound was administered at a concentration of 1 μM.
    Easysep Human Nk Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+nk+cell+isolation+kit/easysep+human+t+cell+isolation+kit/bio_rxiv__2025__07__01__662644-302-13-19
    Average 90 stars, based on 1 article reviews
    easysep human nk cell isolation kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma"

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma

    Journal: bioRxiv

    doi: 10.1101/2025.07.01.662644

    A) Flow cytometry showing GD2 expression levels in the GD2 low cell lines BE2C, NGP and NB1 treated for 14 days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. B) Mean fluorescence intensity for GD2 staining shown in panel A. C-D) Gene expression levels (in TPM from RNA-seq) of B4GALNT1 (C) and ST8SIA1 (D) in BE2C cells treated for six days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. Shown are three biological replicates, and the values represent the mean ± SD. Significance was determined by one-way ANOVA. E) Immunofluorescence for GD2 from FFPE tissue sections of BE2C xenografts in the indicated treatment groups obtained on day 14 of treatment. Scale bar = 20 μm. F-I) Cell viability of BE2C and NB1 cells following both 1) treatment with either DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363 for 14 days and subsequent 2) co-culture with F-G) NK cells and dinutuximab for 24 hours or H-I) GD2 CAR T cells at an effector-to-target (E:T) ratio of 0.5:1 for 48 hours ( n =3 samples per treatment group). Data represent mean values ± SD. Significance was determined by one-way ANOVA. *p<0.05; **p <0.001; ***p <0.001; ****p <0.0001. Each compound was administered at a concentration of 1 μM.
    Figure Legend Snippet: A) Flow cytometry showing GD2 expression levels in the GD2 low cell lines BE2C, NGP and NB1 treated for 14 days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. B) Mean fluorescence intensity for GD2 staining shown in panel A. C-D) Gene expression levels (in TPM from RNA-seq) of B4GALNT1 (C) and ST8SIA1 (D) in BE2C cells treated for six days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. Shown are three biological replicates, and the values represent the mean ± SD. Significance was determined by one-way ANOVA. E) Immunofluorescence for GD2 from FFPE tissue sections of BE2C xenografts in the indicated treatment groups obtained on day 14 of treatment. Scale bar = 20 μm. F-I) Cell viability of BE2C and NB1 cells following both 1) treatment with either DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363 for 14 days and subsequent 2) co-culture with F-G) NK cells and dinutuximab for 24 hours or H-I) GD2 CAR T cells at an effector-to-target (E:T) ratio of 0.5:1 for 48 hours ( n =3 samples per treatment group). Data represent mean values ± SD. Significance was determined by one-way ANOVA. *p<0.05; **p <0.001; ***p <0.001; ****p <0.0001. Each compound was administered at a concentration of 1 μM.

    Techniques Used: Flow Cytometry, Expressing, Fluorescence, Staining, Gene Expression, RNA Sequencing, Immunofluorescence, Co-Culture Assay, Concentration Assay

    Related Articles

    Cell Isolation:

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: B cell , EasySep Human Naïve B Cell Isolation Kit (StemCell Technologies) , RPMI +5% FBS +1% penicillin/streptomycin , Control medium +5 μg/mL anti-CD40 antibody + 20 ng/mL IL-4 , 72 h. .. NK Cell , EasySep Human NK Cell Isolation Kit (StemCell Technologies) , TheraPeak X-VIVO-10 medium (Lonza) + 10% human serum AB+ 1 ng/mL IL-15 , Control medium + 10 ng/mL IL-12 + 50 ng/mL IL-15 + 50 ng/mL IL-18 , 24 h. ..

    Article Title: Transcriptional signature of rapidly responding NK cells reveals S1P5 and CXCR4 as anti-tumor response disruptors.
    Article Snippet: Red blood cells were lysed using RBC lysis buffer (eBioscience, 00-4333-57). .. For NK cell isolation the EasySep Human NK Cell Isolation Kit (STEMCELL, 17955RF) was used. .. NK cells were cultured immediately after isolation in IMDM media (gibco, 12440053) supplemented with 10% HI Sera.

    Article Title: The inhibitory receptor LAG3 affects NK cell IFN-γ production through glycolysis and the PSAT1/STAT1/IFNG pathway
    Article Snippet: .. Negative isolation of NK cells from PBMCs was performed with the EasySep Human NK Cell Isolation kit (Stemcell). .. The sorted NK cells were stimulated with the cytokine mixture for 15 minutes and then immediately treated with BD Fix Buffer I in a 37°C water bath for 10 minutes and Perm Buffer III (BD Biosciences) on ice for 30 minutes, followed by staining with the phosphorylation markers AKT-FITC, mTOR-PE, total STAT1-APC (BD Biosciences), and S6-APC (eBioscience).

    Article Title: The inhibitory receptor LAG3 affects NK cell IFN-γ production through glycolysis and the PSAT1/STAT1/IFNG pathway.
    Article Snippet: .. Analysis of signaling pathway phosphorylation levels Negative isolation of NK cells from PBMCs was performed with the EasySep Human NK Cell Isolation kit (Stemcell). .. The sorted NK cells were stimulated with the cytokine mixture for 15 minutes and then immediately treated with BD Fix Buffer I in a 37°C water bath for 10 minutes and Perm Buffer III (BD Biosciences) on ice for 30 minutes, followed by staining with the phosphorylation markers AKT-FITC, mTOR-PE, total STAT1-APC (BD Biosciences), and S6-APC (eBioscience).

    Article Title: Precision enhancement of CAR-NK cells through non-viral engineering and highly multiplexed base editing.
    Article Snippet: .. CD56+CD3 NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, Massachusetts, USA). .. NK cells were frozen at 2.5E6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, Massachusetts, USA).

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma
    Article Snippet: .. PBMCs were isolated as described above followed by NK cell selection using the EasySep Human NK Cell Isolation Kit (Stemcell technologies) and cultured in NK NK MACS Media (Miltenyi Biotec) supplemented with 500 U/mL recombinant human IL-2 (R&D systems) and 1% penicillin/streptomycin/glutamine. ..

    Article Title: NKG7 is a Stable Marker of Cytotoxicity Across Immune Contexts and Within the Tumor Microenvironment.
    Article Snippet: NK cells were isolated from PBMCs using the EasySep Human NK cell Isolation Kit (STEMCELL Technologies, cat. 17955). .. NK cells were isolated from PBMCs using the EasySep Human NK cell Isolation Kit (STEMCELL Technologies, cat. 17955). ..

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: Peripheral blood mononuclear cells were obtained by Ficoll-Paque (cytiva) density gradient centrifugation. .. Human primary NK cells were isolated from PBMCs using EasySep Human NK Cell Isolation Kit (STEMCELL) and cultured in MEMα medium with 0.1mM β-mercaptoethanol, 12.5% horse serum, 12.5% FBS, 1%P/S and 100 U/mL recombinant human IL-2. ..

    Control:

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: B cell , EasySep Human Naïve B Cell Isolation Kit (StemCell Technologies) , RPMI +5% FBS +1% penicillin/streptomycin , Control medium +5 μg/mL anti-CD40 antibody + 20 ng/mL IL-4 , 72 h. .. NK Cell , EasySep Human NK Cell Isolation Kit (StemCell Technologies) , TheraPeak X-VIVO-10 medium (Lonza) + 10% human serum AB+ 1 ng/mL IL-15 , Control medium + 10 ng/mL IL-12 + 50 ng/mL IL-15 + 50 ng/mL IL-18 , 24 h. ..

    Isolation:

    Article Title: The inhibitory receptor LAG3 affects NK cell IFN-γ production through glycolysis and the PSAT1/STAT1/IFNG pathway
    Article Snippet: .. Negative isolation of NK cells from PBMCs was performed with the EasySep Human NK Cell Isolation kit (Stemcell). .. The sorted NK cells were stimulated with the cytokine mixture for 15 minutes and then immediately treated with BD Fix Buffer I in a 37°C water bath for 10 minutes and Perm Buffer III (BD Biosciences) on ice for 30 minutes, followed by staining with the phosphorylation markers AKT-FITC, mTOR-PE, total STAT1-APC (BD Biosciences), and S6-APC (eBioscience).

    Article Title: The inhibitory receptor LAG3 affects NK cell IFN-γ production through glycolysis and the PSAT1/STAT1/IFNG pathway.
    Article Snippet: .. Analysis of signaling pathway phosphorylation levels Negative isolation of NK cells from PBMCs was performed with the EasySep Human NK Cell Isolation kit (Stemcell). .. The sorted NK cells were stimulated with the cytokine mixture for 15 minutes and then immediately treated with BD Fix Buffer I in a 37°C water bath for 10 minutes and Perm Buffer III (BD Biosciences) on ice for 30 minutes, followed by staining with the phosphorylation markers AKT-FITC, mTOR-PE, total STAT1-APC (BD Biosciences), and S6-APC (eBioscience).

    Article Title: Precision enhancement of CAR-NK cells through non-viral engineering and highly multiplexed base editing.
    Article Snippet: .. CD56+CD3 NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, Massachusetts, USA). .. NK cells were frozen at 2.5E6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, Massachusetts, USA).

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma
    Article Snippet: .. PBMCs were isolated as described above followed by NK cell selection using the EasySep Human NK Cell Isolation Kit (Stemcell technologies) and cultured in NK NK MACS Media (Miltenyi Biotec) supplemented with 500 U/mL recombinant human IL-2 (R&D systems) and 1% penicillin/streptomycin/glutamine. ..

    Article Title: NKG7 is a Stable Marker of Cytotoxicity Across Immune Contexts and Within the Tumor Microenvironment.
    Article Snippet: NK cells were isolated from PBMCs using the EasySep Human NK cell Isolation Kit (STEMCELL Technologies, cat. 17955). .. NK cells were isolated from PBMCs using the EasySep Human NK cell Isolation Kit (STEMCELL Technologies, cat. 17955). ..

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: Peripheral blood mononuclear cells were obtained by Ficoll-Paque (cytiva) density gradient centrifugation. .. Human primary NK cells were isolated from PBMCs using EasySep Human NK Cell Isolation Kit (STEMCELL) and cultured in MEMα medium with 0.1mM β-mercaptoethanol, 12.5% horse serum, 12.5% FBS, 1%P/S and 100 U/mL recombinant human IL-2. ..

    Phospho-proteomics:

    Article Title: The inhibitory receptor LAG3 affects NK cell IFN-γ production through glycolysis and the PSAT1/STAT1/IFNG pathway.
    Article Snippet: .. Analysis of signaling pathway phosphorylation levels Negative isolation of NK cells from PBMCs was performed with the EasySep Human NK Cell Isolation kit (Stemcell). .. The sorted NK cells were stimulated with the cytokine mixture for 15 minutes and then immediately treated with BD Fix Buffer I in a 37°C water bath for 10 minutes and Perm Buffer III (BD Biosciences) on ice for 30 minutes, followed by staining with the phosphorylation markers AKT-FITC, mTOR-PE, total STAT1-APC (BD Biosciences), and S6-APC (eBioscience).

    Selection:

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma
    Article Snippet: .. PBMCs were isolated as described above followed by NK cell selection using the EasySep Human NK Cell Isolation Kit (Stemcell technologies) and cultured in NK NK MACS Media (Miltenyi Biotec) supplemented with 500 U/mL recombinant human IL-2 (R&D systems) and 1% penicillin/streptomycin/glutamine. ..

    Cell Culture:

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma
    Article Snippet: .. PBMCs were isolated as described above followed by NK cell selection using the EasySep Human NK Cell Isolation Kit (Stemcell technologies) and cultured in NK NK MACS Media (Miltenyi Biotec) supplemented with 500 U/mL recombinant human IL-2 (R&D systems) and 1% penicillin/streptomycin/glutamine. ..

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: Peripheral blood mononuclear cells were obtained by Ficoll-Paque (cytiva) density gradient centrifugation. .. Human primary NK cells were isolated from PBMCs using EasySep Human NK Cell Isolation Kit (STEMCELL) and cultured in MEMα medium with 0.1mM β-mercaptoethanol, 12.5% horse serum, 12.5% FBS, 1%P/S and 100 U/mL recombinant human IL-2. ..

    Magnetic Cell Separation:

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma
    Article Snippet: .. PBMCs were isolated as described above followed by NK cell selection using the EasySep Human NK Cell Isolation Kit (Stemcell technologies) and cultured in NK NK MACS Media (Miltenyi Biotec) supplemented with 500 U/mL recombinant human IL-2 (R&D systems) and 1% penicillin/streptomycin/glutamine. ..

    Recombinant:

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma
    Article Snippet: .. PBMCs were isolated as described above followed by NK cell selection using the EasySep Human NK Cell Isolation Kit (Stemcell technologies) and cultured in NK NK MACS Media (Miltenyi Biotec) supplemented with 500 U/mL recombinant human IL-2 (R&D systems) and 1% penicillin/streptomycin/glutamine. ..

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: Peripheral blood mononuclear cells were obtained by Ficoll-Paque (cytiva) density gradient centrifugation. .. Human primary NK cells were isolated from PBMCs using EasySep Human NK Cell Isolation Kit (STEMCELL) and cultured in MEMα medium with 0.1mM β-mercaptoethanol, 12.5% horse serum, 12.5% FBS, 1%P/S and 100 U/mL recombinant human IL-2. ..



    Similar Products

    easysep tm human t cell isolation kit  (Miltenyi Biotec)
    97
    Miltenyi Biotec easysep tm human t cell isolation kit
    Easysep Tm Human T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+nk+cell+isolation+kit/NK+Cell+Isolation+Kit%2C+human/bio_rxiv__2025__09__24__678202-216-13-25
    Average 97 stars, based on 1 article reviews
    easysep tm human t cell isolation kit - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier
    easysep human nk cell isolation kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easysep human nk cell isolation kit
    A) Flow cytometry showing GD2 expression levels in the GD2 low cell lines BE2C, NGP and NB1 treated for 14 days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. B) Mean fluorescence intensity for GD2 staining shown in panel A. C-D) Gene expression levels (in TPM from RNA-seq) of B4GALNT1 (C) and ST8SIA1 (D) in BE2C cells treated for six days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. Shown are three biological replicates, and the values represent the mean ± SD. Significance was determined by one-way ANOVA. E) Immunofluorescence for GD2 from FFPE tissue sections of BE2C xenografts in the indicated treatment groups obtained on day 14 of treatment. Scale bar = 20 μm. F-I) Cell viability of BE2C and NB1 cells following both 1) treatment with either DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363 for 14 days and subsequent 2) co-culture with F-G) <t>NK</t> <t>cells</t> and dinutuximab for 24 hours or H-I) GD2 CAR T cells at an effector-to-target (E:T) ratio of 0.5:1 for 48 hours ( n =3 samples per treatment group). Data represent mean values ± SD. Significance was determined by one-way ANOVA. *p<0.05; **p <0.001; ***p <0.001; ****p <0.0001. Each compound was administered at a concentration of 1 μM.
    Easysep Human Nk Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+nk+cell+isolation+kit/easysep+human+t+cell+isolation+kit/bio_rxiv__2025__07__01__662644-302-13-19
    Average 90 stars, based on 1 article reviews
    easysep human nk cell isolation kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    easysep negative human nk cell isolation kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easysep negative human nk cell isolation kit
    TRPM3 ion channel function in <t>NK</t> <t>cells</t> modulated with PregS and ononetin. (A–C) Current obtained on an <t>NK</t> <t>cell</t> from an HC participant. (A) A representative time-series of current amplitude at +100 mV and −100 mV. (B) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (C) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (D–F) Current obtained on an NK cell from a long COVID participant. (D) A representative time-series of current amplitude at +100 mV and −100 mV. (E) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (F) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (G–I) - Current obtained on an NK cell from a long COVID participant on treatment with LDN. (G) A representative time-series of current amplitude at +100 mV and −100 mV. (H) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (I) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. Dash lines in (A,D,G) illustrate each trend of the baseline and PregS effects. Abbreviations: HC, healthy control; LC, long COVID; LDN, low-dose naltrexone; NK, natural killer; PregS, pregnenolone sulfate.
    Easysep Negative Human Nk Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+nk+cell+isolation+kit/easysep+human+t+cell+isolation+kit/pmc12127304-103-12-19
    Average 90 stars, based on 1 article reviews
    easysep negative human nk cell isolation kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    easysep human nk-cell isolation kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easysep human nk-cell isolation kit
    TRPM3 ion channel function in <t>NK</t> <t>cells</t> modulated with PregS and ononetin. (A–C) Current obtained on an <t>NK</t> <t>cell</t> from an HC participant. (A) A representative time-series of current amplitude at +100 mV and −100 mV. (B) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (C) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (D–F) Current obtained on an NK cell from a long COVID participant. (D) A representative time-series of current amplitude at +100 mV and −100 mV. (E) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (F) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (G–I) - Current obtained on an NK cell from a long COVID participant on treatment with LDN. (G) A representative time-series of current amplitude at +100 mV and −100 mV. (H) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (I) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. Dash lines in (A,D,G) illustrate each trend of the baseline and PregS effects. Abbreviations: HC, healthy control; LC, long COVID; LDN, low-dose naltrexone; NK, natural killer; PregS, pregnenolone sulfate.
    Easysep Human Nk Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+nk+cell+isolation+kit/easysep+human+t+cell+isolation+kit/pmc12051578-60-21-27
    Average 90 stars, based on 1 article reviews
    easysep human nk-cell isolation kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Flow cytometry showing GD2 expression levels in the GD2 low cell lines BE2C, NGP and NB1 treated for 14 days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. B) Mean fluorescence intensity for GD2 staining shown in panel A. C-D) Gene expression levels (in TPM from RNA-seq) of B4GALNT1 (C) and ST8SIA1 (D) in BE2C cells treated for six days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. Shown are three biological replicates, and the values represent the mean ± SD. Significance was determined by one-way ANOVA. E) Immunofluorescence for GD2 from FFPE tissue sections of BE2C xenografts in the indicated treatment groups obtained on day 14 of treatment. Scale bar = 20 μm. F-I) Cell viability of BE2C and NB1 cells following both 1) treatment with either DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363 for 14 days and subsequent 2) co-culture with F-G) NK cells and dinutuximab for 24 hours or H-I) GD2 CAR T cells at an effector-to-target (E:T) ratio of 0.5:1 for 48 hours ( n =3 samples per treatment group). Data represent mean values ± SD. Significance was determined by one-way ANOVA. *p<0.05; **p <0.001; ***p <0.001; ****p <0.0001. Each compound was administered at a concentration of 1 μM.

    Journal: bioRxiv

    Article Title: KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma

    doi: 10.1101/2025.07.01.662644

    Figure Lengend Snippet: A) Flow cytometry showing GD2 expression levels in the GD2 low cell lines BE2C, NGP and NB1 treated for 14 days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. B) Mean fluorescence intensity for GD2 staining shown in panel A. C-D) Gene expression levels (in TPM from RNA-seq) of B4GALNT1 (C) and ST8SIA1 (D) in BE2C cells treated for six days with DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363. Shown are three biological replicates, and the values represent the mean ± SD. Significance was determined by one-way ANOVA. E) Immunofluorescence for GD2 from FFPE tissue sections of BE2C xenografts in the indicated treatment groups obtained on day 14 of treatment. Scale bar = 20 μm. F-I) Cell viability of BE2C and NB1 cells following both 1) treatment with either DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363 for 14 days and subsequent 2) co-culture with F-G) NK cells and dinutuximab for 24 hours or H-I) GD2 CAR T cells at an effector-to-target (E:T) ratio of 0.5:1 for 48 hours ( n =3 samples per treatment group). Data represent mean values ± SD. Significance was determined by one-way ANOVA. *p<0.05; **p <0.001; ***p <0.001; ****p <0.0001. Each compound was administered at a concentration of 1 μM.

    Article Snippet: PBMCs were isolated as described above followed by NK cell selection using the EasySep Human NK Cell Isolation Kit (Stemcell technologies) and cultured in NK NK MACS Media (Miltenyi Biotec) supplemented with 500 U/mL recombinant human IL-2 (R&D systems) and 1% penicillin/streptomycin/glutamine.

    Techniques: Flow Cytometry, Expressing, Fluorescence, Staining, Gene Expression, RNA Sequencing, Immunofluorescence, Co-Culture Assay, Concentration Assay

    TRPM3 ion channel function in NK cells modulated with PregS and ononetin. (A–C) Current obtained on an NK cell from an HC participant. (A) A representative time-series of current amplitude at +100 mV and −100 mV. (B) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (C) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (D–F) Current obtained on an NK cell from a long COVID participant. (D) A representative time-series of current amplitude at +100 mV and −100 mV. (E) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (F) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (G–I) - Current obtained on an NK cell from a long COVID participant on treatment with LDN. (G) A representative time-series of current amplitude at +100 mV and −100 mV. (H) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (I) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. Dash lines in (A,D,G) illustrate each trend of the baseline and PregS effects. Abbreviations: HC, healthy control; LC, long COVID; LDN, low-dose naltrexone; NK, natural killer; PregS, pregnenolone sulfate.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Low‐Dose naltrexone restored TRPM3 ion channel function in natural killer cells from long COVID patients

    doi: 10.3389/fmolb.2025.1582967

    Figure Lengend Snippet: TRPM3 ion channel function in NK cells modulated with PregS and ononetin. (A–C) Current obtained on an NK cell from an HC participant. (A) A representative time-series of current amplitude at +100 mV and −100 mV. (B) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (C) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (D–F) Current obtained on an NK cell from a long COVID participant. (D) A representative time-series of current amplitude at +100 mV and −100 mV. (E) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (F) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. (G–I) - Current obtained on an NK cell from a long COVID participant on treatment with LDN. (G) A representative time-series of current amplitude at +100 mV and −100 mV. (H) I–V curve before (baseline) and after TRPM3 stimulation through PregS application. (I) I–V curve on PregS stimulation and after TRPM3 inhibition with ononetin in the presence of PregS. Dash lines in (A,D,G) illustrate each trend of the baseline and PregS effects. Abbreviations: HC, healthy control; LC, long COVID; LDN, low-dose naltrexone; NK, natural killer; PregS, pregnenolone sulfate.

    Article Snippet: Immediately after PBMC isolation, NK cells were isolated using immunomagnetic selection and EasySep Negative Human NK Cell Isolation Kit (Stem Cell Technologies, Vancouver, BC, Canada).

    Techniques: Inhibition, Control

    TRPM3 ion channel function comparison among long COVID, HC and long COVID taking LDN groups. (A) Bar graph showing TRPM3 current amplitude at +100 mV after TRPM3 stimulation through PregS application in long COVID (N = 9; n = 61), HC (N = 9; n = 65) and long COVID taking LDN (N = 9; n = 63). (B) Bar graphs illustrating TRPM3 inhibition with ononetin in the presence of PregS in long COVID (N = 9; n = 52), HC (N = 9; n = 53) and long COVID taking LDN (N = 9; n = 53). (C) Bar graphs representing the percentage of NK cells sensitive and insensitive to ononetin inhibition in the presence of PregS. (D–F) Scatter plots showing change of each current recording in NK cells from long COVID, HC and long COVID taking LDN groups, respectively; first point was measured at the end of TRPM3 stimulation through PregS application, and the second point shows the effect in current amplitude after TRPM3 inhibition with ononetin in the presence of PregS. Each red line represented a cell sensitive to ononetin as a reduction in amplitude was recorded. N = participants and n = number of records. Data presented as mean ± SEM and determined by Kruskal–Wallis (Dunn’s multiple comparisons, (A,B) in amplitude analysis and Fisher’s exact test (Bonferroni method, C) regarding ononetin response. Abbreviations: HC, healthy control; LC, long COVID; LDN, low-dose naltrexone; N, number of participants; n, number of records analyzed; NK, natural killer; PregS, pregnenolone sulfate.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Low‐Dose naltrexone restored TRPM3 ion channel function in natural killer cells from long COVID patients

    doi: 10.3389/fmolb.2025.1582967

    Figure Lengend Snippet: TRPM3 ion channel function comparison among long COVID, HC and long COVID taking LDN groups. (A) Bar graph showing TRPM3 current amplitude at +100 mV after TRPM3 stimulation through PregS application in long COVID (N = 9; n = 61), HC (N = 9; n = 65) and long COVID taking LDN (N = 9; n = 63). (B) Bar graphs illustrating TRPM3 inhibition with ononetin in the presence of PregS in long COVID (N = 9; n = 52), HC (N = 9; n = 53) and long COVID taking LDN (N = 9; n = 53). (C) Bar graphs representing the percentage of NK cells sensitive and insensitive to ononetin inhibition in the presence of PregS. (D–F) Scatter plots showing change of each current recording in NK cells from long COVID, HC and long COVID taking LDN groups, respectively; first point was measured at the end of TRPM3 stimulation through PregS application, and the second point shows the effect in current amplitude after TRPM3 inhibition with ononetin in the presence of PregS. Each red line represented a cell sensitive to ononetin as a reduction in amplitude was recorded. N = participants and n = number of records. Data presented as mean ± SEM and determined by Kruskal–Wallis (Dunn’s multiple comparisons, (A,B) in amplitude analysis and Fisher’s exact test (Bonferroni method, C) regarding ononetin response. Abbreviations: HC, healthy control; LC, long COVID; LDN, low-dose naltrexone; N, number of participants; n, number of records analyzed; NK, natural killer; PregS, pregnenolone sulfate.

    Article Snippet: Immediately after PBMC isolation, NK cells were isolated using immunomagnetic selection and EasySep Negative Human NK Cell Isolation Kit (Stem Cell Technologies, Vancouver, BC, Canada).

    Techniques: Comparison, Inhibition, Control